Laser-Capture Microdissection Facility
While high throughput Genomic/Proteomic technologies are rapidly advancing our understanding of complex gene/protein interactions, they function best when used on homogenous cell systems. Unfortunately most organ systems are complex amalgams of tissues composed of multiple cell types. This results in an averaging of expression data across the various cell types and leads to greater confusion in attempts to analyze the data from these technologies. With the advent of more sensitive detection systems and amplification technologies smaller quantities of material are necessary to yield interpretable results. Laser-Capture Microdissection allows the investigator to select specific regions within a section of tissue down to a few cells.The equipment is available to all AECOM investigators in blocks of two hours. The facility can provide consultations on projects, grant support material and training for users.
Equipment: The facility consists of a Leica AS LMD laser capture microdissection system with 2.5X, 4X, 10X, 40X and 100X objectives
with florescence capacity for red, green, yellow and cyan flurophores.
A Hitachi HV-C20A color CCD provides image capture capacity, and the entire system is controlled by a Dell Optiplex GX240 computer
Using Windows Based software the area(s) of interest can be selected, labeled and cut. The system allows image files to be saved in multiple formats to document the procedure.
All new users must schedule an appointment for a training session and system logon. For scheduling, consultations or training please contact the faculty director Dr. Thomas Harris
Useful Files: Facility usage Guide:
HISTOTECHNOLOGY AND COMPARATIVE PATHOLOGY FACILITY Home Page
